Please fill out the form to access and download the poster. Scaling throughput or volume in genetic modification of patient-derived cells can be challenging. Viability and functionality of the sensitive, primary human cells can be at risk and transfection efficiencies can drop. A stable process through a reliable platform and protocol are important.
Find out from this study how our scientists have optimized transfection and culture protocols for primary human T cells by scaling the process from about 1 million cells in 20 μL to 1 billion in 20 mL using the 4D-Nucleofector
® Platform.
The study shows:
- Comparable high transfection efficiencies across all scales
- Proof of concept for both cryopreserved and freshly isolated cells
- Efficient expansion post transfection
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